![]() Yuzbashev TV, Vybornaya TV, Larina AS, Gvilava IT, Voyushina NE, Mokrova SS, Yuzbasheva EY, Manukhov IV, Sineoky SP, Debabov VG (2013) Directed modification of Escherichia coli metabolism for the design of threonine-producing strains. doi: 10.1111/1751-7915.12248Įggeling L, Bott M (2015) A giant market and a powerful metabolism: L-lysine provided by Corynebacterium glutamicum. Keywordsīott M (2015) Need for speed – finding productive mutations using transcription factor-based biosensors, fluorescence-activated cell sorting and recombineering. In this chapter, selected new technologies relevant for breeding microbial production strains with a special emphasis on amino acid producers will be summarized. In particular, next-generation sequencing technologies combined with new methods of genome engineering and high-throughput screening based on genetically encoded biosensors have allowed for new concepts. Starting from classical strain breeding by random mutagenesis and screening in the 1950s via rational design by metabolic engineering initiated in the 1970s, a range of powerful new technologies have been developed in the past two decades that can revolutionize future strain engineering. ![]() The implementation of a knowledge-based bioeconomy requires the rapid development of highly efficient microbial production strains that are able to convert renewable carbon sources to value-added products, such as bulk and fine chemicals, pharmaceuticals, or proteins at industrial scale.
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